Iodine in rat alveolar macrophages following amiodarone treatment: Quantitative X-ray microanalysis
Identifieur interne : 003199 ( Main/Exploration ); précédent : 003198; suivant : 003200Iodine in rat alveolar macrophages following amiodarone treatment: Quantitative X-ray microanalysis
Auteurs : R. Gary Kirk [États-Unis] ; Mark J. Reasor [États-Unis] ; Ping Lee [États-Unis]Source :
- Experimental and Molecular Pathology [ 0014-4800 ] ; 1988.
English descriptors
- Teeft :
- Academic press, Alveolar macrophages, Amiodarone, Amiodarone treatment, Amorphous, Amorphous bodies, Antiarrhythmic drug, Cationic amphiphilic drugs, Control cells, Control rats, Cytosol, Dense granules, Distinct groups, Electron microscope, Granule, High concentrations, High iodine, High iron, High level, Hostetler, Iodine signal, Kirk, Lamellar, Lamellar bodies, Macrophage, Many cells, Microanalysis, Phospholipid, Phosphorus, Phosphorus signal, Present study, Pulmonary toxicity, Reasor, Same cells, Single administration, Single dose, Subcellular level, Toxicity, Vacuum evaporator.
Abstract
Abstract: The techniques of cryomicrotomy and X-ray microanalysis were used to quantitatively measure the subcellular distribution of iodine in rat alveolar macrophages following a single administration of the iodine-containing antiarrhythmic drug, amiodarone. When frozen, dried sections were analyzed, small amounts of iodine were found throughout the alveolar macrophages, but the major accumulations were observed in amorphous bodies and dense granules. The highest to lowest accumulation is in the following order: amorphous bodies (90 mmole I/kg dry wt) dense granules (50 mmole I/kg dry wt) nucleus = cytosol (10 mmoles I/kg dry wt). The amorphous bodies can contain high and low levels of iodine and the granules are found to have high and low levels of iron. Granules with the high and low levels of iron and amorphous bodies with the high and low levels of iodine can be found in the same cells. X-ray microanalysis proved useful in describing the intracellular distribution of iodine-labeled species following amiodarone administration.
Url:
DOI: 10.1016/0014-4800(88)90006-8
Affiliations:
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Gary Kirk</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie-Occidentale</region></placeName><wicri:cityArea>Department of Anatomy, West Virginia University Health Science Center, Morgantown</wicri:cityArea></affiliation></author><author><name sortKey="Reasor, Mark J" sort="Reasor, Mark J" uniqKey="Reasor M" first="Mark J." last="Reasor">Mark J. 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When frozen, dried sections were analyzed, small amounts of iodine were found throughout the alveolar macrophages, but the major accumulations were observed in amorphous bodies and dense granules. The highest to lowest accumulation is in the following order: amorphous bodies (90 mmole I/kg dry wt) dense granules (50 mmole I/kg dry wt) nucleus = cytosol (10 mmoles I/kg dry wt). The amorphous bodies can contain high and low levels of iodine and the granules are found to have high and low levels of iron. Granules with the high and low levels of iron and amorphous bodies with the high and low levels of iodine can be found in the same cells. X-ray microanalysis proved useful in describing the intracellular distribution of iodine-labeled species following amiodarone administration.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Virginie-Occidentale</li></region></list><tree><country name="États-Unis"><region name="Virginie-Occidentale"><name sortKey="Gary Kirk, R" sort="Gary Kirk, R" uniqKey="Gary Kirk R" first="R." last="Gary Kirk">R. Gary Kirk</name></region><name sortKey="Lee, Ping" sort="Lee, Ping" uniqKey="Lee P" first="Ping" last="Lee">Ping Lee</name><name sortKey="Reasor, Mark J" sort="Reasor, Mark J" uniqKey="Reasor M" first="Mark J." last="Reasor">Mark J. Reasor</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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